Document 0574
 DOCN  M9480574
 TI    Cytotoxic T lymphocyte activity and CD8 subpopulations in children at
       risk of HIV infection.
 DT    9410
 AU    Aldhous MC; Watret KC; Mok JY; Bird AG; Froebel KS; University
       Department of Medicine, Royal Infirmary, Edinburgh,; UK.
 SO    Clin Exp Immunol. 1994 Jul;97(1):61-7. Unique Identifier : AIDSLINE
       MED/94306754
 AB    HIV-specific cytotoxic T lymphocytes (CTL) are thought to play a major
       role in viral control in HIV-infected adults. Changes in the relative
       proportions of CD8 lymphocyte subpopulations are also thought to be
       associated with disease progression. Less is known about the relative
       effectiveness of CTL against different HIV targets, or about the
       relationship, if any, between CTL activity and CD8 subpopulations. We
       have measured CTL activity against four HIV gene products (gag, tat, pol
       and env) and expression of CD45RO, CD45RA, HLA-DR, CD29, S6F1, and CD57
       surface markers on CD8 cells from nine HIV-infected and 11
       HIV-uninfected children. Of nine HIV-infected children, six showed
       antigen-specific CTL activity on at least one occasion: 4/6 directed
       against tat, 6/6 against pol, 1/6 against env, and 1/6 against gag.
       However, the specificity of the CTL activity varied between children and
       within individual children with time. Furthermore, two uninfected
       children showed CTL activity, one to HIV-gag, -pol and -tat, and the
       other to HIV-pol. All the HIV-infected and two uninfected children had
       abnormal proportions of CD8 subpopulations in whole blood compared with
       age-matched controls. There was no correlation between CTL activity and
       CD8 subsets in whole blood. Five children changed from CTL-positive to
       CTL-negative (or vice versa) during the study. In these, the occasions
       when CTL activity was detected coincided with an increase in CD8 cells,
       an expansion of HLA-DR+ CD8 cells and a loss of CD45RA+ CD8 cells.
 DE    Antigens, CD8/*METABOLISM  Antigens, Differentiation,
       T-Lymphocyte/METABOLISM  Child  Child, Preschool  Cytotoxicity,
       Immunologic  Female  Human  HIV Antigens/IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY/TRANSMISSION  In Vitro  Infant  Maternal-Fetal
       Exchange/IMMUNOLOGY  Pregnancy  Risk Factors  Support, Non-U.S. Gov't
       T-Lymphocyte Subsets/*IMMUNOLOGY  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY
       JOURNAL ARTICLE

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